Romana Jassat matriculated at her local State Secondary School in her hometown of  Roshnee, Vereeniging in 2007. She achieved nine distinctions. She completed MBBCh at the University of Natal (UKZN) in 2012. Romana did her community service at Sebokeng Regional Hospital in the Vaal. She then specialized in Internal Medicine at Wits University in 2021, graduating as a physician whilst obtaining a Masters in Medicine (with distinction) during this period.

After spending six months as a volunteer at the haematology unit at Chris Hani Baragwanath Academic Hospital in Johannesburg, Romana received the Bristol Myers Squibb Foundation Fellowship Award in Clinical Haematology. She spent the next 2½ years as a fellow in training in the Wits University circuit, rotating through clinical haematology as well as various aspects of laboratory based haematopathology. She was privileged to be trained in stem cell transplantation at Alberts Cellular Therapy (ACT) in the last phase of her fellowship program.

In October 2024, Romana joined Alberts Cellular Therapy (ACT) based at Netcare Pretoria East Hospital. Romana also consults weekly at the ABJ oncology practice at Mediclinic Vereeniging.