Dr Isoline Botha completed her MBChB studies at the University of Pretoria in 1984. After spending a few years working in rural hospitals in Limpopo Province and in Private Practice in Makhado, she obtained an M Fam Med degree from the UOFS. She then worked in Medical Outpatients for a few years, treating mostly geriatric patients. She spent 9 years working in an oncology department in the public sector.

In August 2008 she obtained a Diploma in Anaesthesia and spent 2 years working in that department. In June 2011 she joined the ABJ practice. Her focus is on supportive care, palliation, and a holistic approach.